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The sample is positioned in the Teflon cup assemblies.
White gel-like precipitation was found deposited on the bottom of the Teflon cup.
After magnetic stirring for 30 min, the Teflon cup was held into stainless steel sealing autoclave and heated to 200 °C for 16 h.
Approximately 0.50 g of this material, were weighed accurately into a Teflon cup, and dissolved in concentrated nitric acid (~10 mL), with heating in a water bath.
According to the ratio of BaY1-x-yF5:xEr3+, yYb3+, the solution of Ba(OH 2·xH2O, Y NO3 3·6H2O, (CH3CO2 3Er, Yb NO3 3, and NaBF4 were put into a Teflon cup.
The mixture was transferred into a Teflon cup.
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Sample aliquots of 10 ml were placed into 10 separate acid-washed Teflon cups (Savillex™).
The sulfuric acid pretreatment was conducted in a batch mode using mineralization bombs equipped with Teflon cups (Parr).
Two of the Teflon cups contained no added copper, and the remaining eight cups contained 1, 2.5, 5, 8, 15, 25, 50, and 100 nM copper (0.06, 0.16, 0.32, 0.51, 0.95, 1.59, 3.18, and 6.36 μg 1−1) to titrate the naturally occurring organic copper-binding ligands.
The Si substrate was placed together into a teflon pressure cooker with a cup of 0.2 ml octadecyltrimethoxysilane (ODS) and sealed with a cap.
Resulting cells were suspended in RPMI 1640 complete medium containing 2 mM L-glutamine and 20% human serum (Gemini Bioproducts), transferred into Teflon-coated tissue culture cups (Savillex), and incubated for 5 days to allow for differentiation of monocytes into macrophages.
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