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It has been recognized that tissue engineering and regenerative medicine (TERM) offers the next generation technology for whole organ and tissue transplantation for diseased, failed or malfunctioned organs.
Development of advance technology for whole genome expression studies is thus becoming increasingly recognized.
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But a technology for whole-genome analysis of microbes could lead to quicker identification and more comprehensive analysis.
If NGS technology for whole-genome sequencing were applied in these cases, more information and accuracy would be obtained.
High-density oligonucleotide arrays are currently the most widely used high-throughput technology for whole-genome gene expression studies.
For example, several groups are utilizing next generation sequencing technology for whole-genome sequencing of primary tumors and matched metastases [ 63, 64].
Furthermore, we have demonstrated the effectiveness of the bovine SNP chip technology for whole-genome association analyses and elucidation of causative genetic mutations, even when relatively few samples are available.
In this perspective, a high-throughput genome-wide QTL-seq strategy that employs NGS technology for whole-genome resequencing of two DNA bulks of progeny from a segregating mapping population with extremely contrasting phenotypic trait values seems quite pertinent.
Standardization of sequences for bone metastasis assessment by whole-body diffusion MR imaging and improvement of MR technology for whole-body examination are necessary for better results in the future.
There is currently no high-throughput technology for whole-transcriptome analysis that preserves spatial coordinates, and while pioneering studies can be conceived in transgenic models expressing spatially controlled reporter genes, strategies must be prepared for the study of human tissues, where this method will not be available.
Genotyping technologies for whole genome association studies are now available.
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