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The thickness of H&E stained epidermis was measured without the stratum corneum on 66 randomly chosen areas between the hair follicles on scanned slides using digital image analysis software (Image Scope, Aperio Technologies, version 10.2).. Mast cells stained with toluidine blue and eosinophils stained with congo red were counted as described [25].
The macrophage transcriptional profile of the 6 donors (being stimulated or not, by LPS) was evaluated independently using the Operon Human Genome Array-Ready Oligo Set™ (AROS, Operon Technologies) Version 4.0, containing 35,035 oligonucleotide probes representing approximately 25,100 unique genes and 39,600 transcripts.
Features were extracted with an image analysis tool (Agilent Technologies, version A 6.1.1) using default settings.
Data analysis was performed using the GeneSpring GX software (Agilent Technologies, version 7.3).
The mass spectrometry raw data was processed to peak list format by using MassHunter Qualitative Analysis software (Agilent Technologies, Version B.03.3).
Peaks were quantified using MSD Chemstation software (Agilent Technologies, version E.02.00.493), using ion 57 as the target ion to quantify the abundance of each CHC compound.
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