Exact(4)
The ability to link imaging to biology for guiding therapy should improve the rate at which novel imaging technologies, probes, contrast agents, drugs and drug delivery systems can be transferred into clinical practice.
For samples processed for 454 and Ion Torrent technologies, probes for bacterial ribosomal RNAs from the Ribominus kit (Invitrogen) were added during the subtractive hybridization procedure.
Each F2 population was fixed and prepared for FISH as previously described (Kowalinski et al., 2011) using custom Stellaris (Biosearch Technologies) probes for the Orsay virus RNA1 molecule (Supplementary file 1B) labeled with Quasar 670 Dye.
As for other microarray technologies, probes designed for Affymetrix gene expression assay are biased towards the 3' end of the transcripts: the length of the target is therefore not a critical metric provided the targets cover the first 700 bases from the 3' end of the transcripts.
Similar(56)
Emerging fluorescence-based technologies probe in vivo binding equilibrium and stoichiometry of protein complexes.
The antigens found for the various cancers analyzed so far by proteome serological analyses and by SEREX show very little overlap suggesting that these two technologies probe different categories of antigens and may complement each other for the elucidation of the antigenicity of tumor cells.
The methodology we have developed here for detecting rare variant associations is extremely simplistic, and as technologies probing human genome sequence variation move rapidly forward, the development and testing of analytical strategies that maximise output from these investments will continue to be of critical importance.
In a participatory design process enhanced with technology probes, we individually discussed potential solutions for specific homes with 14 people.
The design of the system was informed by an iterative process of developing, deploying and evaluating a set of three technology probes.
Our next study, for example, will involve care dyads to codesign "technology probes" [ 31] that can then be deployed and longitudinally studied in real-world home settings.
Continued development of new technology, probes and assays should help in the better understanding of basic breast cancer biology and in the improved management of breast cancer patients.
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