Methods for HLA typing are described, including serological methods, molecular techniques of sequence-specific priming (SSP), sequence-specific oligonucleotide probing (SSOP), Sequence based typing (SBT) and reference strand-based conformation analysis (RSCA) method.
The term "exome" may no longer be appropriate for a technique that is simply a subset of the more generic technique of sequence capture.
Using the technique of sequence analysis of sodium bisulphite-treated genomic DNA (Clark et al, 1994), we find a strong correlation between hypermethylation of the Testisin 5′ region and loss of Testisin mRNA expression both in human tumour cell lines and in primary testicular tumour tissues.
DNA barcoding refers to the technique of sequencing a short fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene from a taxonomically unknown specimen and performing comparisons with a reference library of sequences of known species origin to establish a species-level identification.
In the early 80s, when Sulston had finished the embryonic lineage of the worm, the techniques of gene sequencing were still in their infancy.
Her son, Dr. McDaniel, decided he would orchestrate the use of the most advanced techniques of gene sequencing and analysis to take on her cancer.
Overall, the relatively newer techniques of bisulfite sequencing and cassette methylation greatly augment the utility of the older techniques of 5-azaC treatment and DNaseI hypersensitivity analyses.
The novel techniques of aDNA sequencing have enabled the identification of genes related to susceptibility and resistance phenotypes within ancient populations, which may be an important factor in the determination of past bacterial prevalence.
Although fast and efficient techniques of DNA sequencing and proteomics are available today and the detailed analysis of genome structure and organisation is possible, there is not much molecular data about Klebsiella virulent bacteriophages.
The development of techniques for sequencing deoxyribonucleic acid (DNA) from environmental samples was a crucial factor for the discovery of the exceptional degree of diversity among prokaryotes.
