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The mortality associated with this procedure has decreased rapidly in the past decades due to refinements in operative technique, introduction of new surgical devices, and improvements in postoperative care, including new interventional radiology techniques; however, morbidity remains high [ 1– 6].
Today, genetic modification of the Ti plasmid is one of the main techniques for introduction of transgenes to plants and the creation of genetically modified crops.
These ideas are the basis of HTES and AHS techniques (see "Introduction" section).
Achieving better outcomes depends both on the advancements in microsurgical techniques and introduction of molecular biology discoveries into clinical practice.
Key advances include: (i) progress in oligonucleotide synthesis and in vitro and in vivo assembly methods, (ii) optimization of recombineering techniques, (iii) introduction of parallel, large-scale, combinatorial, and automated genome modification procedures, and (iv) rapid identification of the modifications by barcode-based analysis and sequencing.
According to the authors of the IC3D classification system (the International Committee for Classification of Corneal Dystrophies), understanding of corneal dystrophies is still evolving due to the development of noninvasive imaging techniques and introduction of genetic testing [ 26].
Although LDF might be replaced in future by more sensitive and robust laser Doppler imaging techniques, and introduction of LDF into routine clinical care is rather unlikely for several reasons, data on "normal" endothelial function in healthy children is a prerequisite for counseling of patients participating in experimental research.
The final negotiation involved another time-tested technique: the introduction of an outsider that the militants trust, in this case Nevada assemblywoman Michele Fiore, to act as a go-between.
In the open-entry technique, the introduction of sharp instruments is avoided.
In such cases, a modified exchange technique with introduction of the new THC through a remote exit site and the preexisting subcutaneous tunnel may be a solution.
In the second part, shrinkage porosity analysis is performed using casting simulation technique by introduction of a new gating system designed, solid model developed for four cavities mould.
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