Using our protocol, 17 putative transgenic plants expressing artificial microRNAs that target Cucumber mosaic virus (CMV) 2a/2b genes were recovered, and seven of them were succeeded in obtaining T1 generation seeds because of the technique difficulties of self-pollination of the transgenic watermelon lines.
This discrepancy may be the result of the inherent technique difficulties of RT-PCR analysis, which we have previously addressed [21].
We therefore failed to find the CIP2A targets in JNK signaling pathway due to the technique difficulties.
He talked in a way I had never come across before, without any reserve or hesitation, roaming across all sorts of subjects: verse techniques, the difficulty of finding servants, staying with Max Beerbohm, the Test series, his first meeting with Thomas Hardy, the shortcomings of his wife/son/daughter-in-law, his neglect by the critics - this last a recurring theme.
Most existing indexing techniques have difficulty dealing with the document query due to high dimensionality and sparse representation of a document query.
However, software-based registration techniques have difficulty accounting for differences in patient positioning and involuntary movement of internal organs, often necessitating labor-intensive nonlinear mapping that may not converge to a satisfactory result.
Current clinical imaging processing techniques have difficulty in reconstructing accurate 3D models for small vessels (e.g., with a diameter less than 0.5 mm) owing to the limitation of the imaging resolution.
Both techniques have difficulties regarding accuracy of the measurements at the ends of their energy ranges.
Nevertheless, traditional data mining techniques find difficulties in their application on current scenarios, due to the complexity previously mentioned.
Traditional physical deposition techniques have difficulties in controlling the particle size and morphology due to the random nucleation during the initial growth stage.
