Furthermore, methodological differences in qPCR techniques (different primers and reference genes) or the composition of patient cohorts (different GS subgroups) may further explain discrepancies in marker detection.
There are several explanations for the discrepancy in the number of HLA class I negative tumors between the study of Watson et al. and ours, like different definition of HLA class I expression, differences in staining techniques, different patient cohort and number of MSI tumors.
Inconsistent results can arise from use of novel techniques, different test systems, uncertainty and differences in test chemical composition and purity, and a myriad of other factors.
Consequently, by combining these techniques, different retrofit strategies were proposed.
Writing a libretto demands techniques different from those for writing spoken drama.
Regarding the extraction techniques, different approaches previously optimized in the research group were tested.
Emphasis is given in the utilization of various SHM techniques, different modeling philosophies (data-driven/physics based) and machine learning algorithms.
The picture is sometimes obscured by the way the grapes are grown (vineyard yields and the amount of shading) and cellar techniques (different yeasts, contact with the skins and fermentation temperatures), but the distinctions hold.
