The immunohistochemical technique, assessment of expression and identifications of groups of high vs low HIF or CA9 reactivity used have been described in previous studies (Giatromanolaki et al, 2001a, 2001b).
The main strengths of our study include the use of a wide range of techniques for expression analysis and the comparison between WTs and matched-paired NKs to reduce the confounding variation inherent in biological samples.
HPV positive and negative tumours were investigated using standard immunohistochemical techniques for expression of the Bak protein, the proliferation marker Ki-67 and apoptosis as judged by TUNEL staining.
The trope turns the play into a cleverly dramatized seminar on seeing: abstraction vs. representation, technique vs. quality of expression, vision vs. decoration.
Indeed, the movement comprised many different painterly styles varying in both technique and quality of expression.
Huey said Metallica expanded its compositional technique and range of expression to take on a more aggressive approach in following releases, and lyrics dealt with personal and socially conscious issues.
The data show that this high-throughput protein profiling technique identifies patterns of expression that discriminate different types of breast tumors that group according to clinicopathological variables, and provides similar classification groups to cDNA expression profiling.
Besides cell-specific and treatment-specific studies by mutagenesis strategy, to identify genes of potential importance to stresses stimulates investigators to utilize high-throughput techniques of global expression profiling which can reveal transcriptional changes on a genome-wide scale [65].
We cannot then discard the possibility that newer and more reliable techniques of gene expression detection could produce different results.
Novel techniques of gene expression and proteomic analysis have been applied to obtain a better understanding of the molecular events underlying trauma or genetically-induced degeneration.
Unconstrained by the rigours of conventional manufacturing techniques, this freedom of expression is expected to lead to a host of advances that will make power generation both more efficient and lower cost.
