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Moreover, SBB is a technique employable in frozen tissue [ 27], so it is also ideal for use in parallel with SA-β-gal as an additional biomarker of the senescent state.
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With the aim to produce, by a simple and reproducible technique, porous scaffolds potentially employable for tissue engineering purposes, in this work, we have synthesized a methacrylate (MA) copolymer of α,β-poly N-2-hydroxyethyl -dl-aspartamide (PHEA) and polylactic acid (PLA).
While this study utilized ears that had been clipped for ease of experimental study (minimizing restraint, anesthesia, etc)., the Raman technique is easily made portable and employable via fiber optic means, which would allow its noninvasive interrogation of the tissue to be utilized in clinical environments.
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