Using our protocol, 17 putative transgenic plants expressing artificial microRNAs that target Cucumber mosaic virus (CMV) 2a/2b genes were recovered, and seven of them were succeeded in obtaining T1 generation seeds because of the technique difficulties of self-pollination of the transgenic watermelon lines.
This discrepancy may be the result of the inherent technique difficulties of RT-PCR analysis, which we have previously addressed [21].
Thus, such a technique avoids the difficulties of performing inference on mixtures, since the mixtures only model instantaneous distributions.
These techniques overcome the difficulties of classical statistical methods such as spatial correlation, non-linearity of data, and overfitting (Were et al. 2015).
He talked in a way I had never come across before, without any reserve or hesitation, roaming across all sorts of subjects: verse techniques, the difficulty of finding servants, staying with Max Beerbohm, the Test series, his first meeting with Thomas Hardy, the shortcomings of his wife/son/daughter-in-law, his neglect by the critics - this last a recurring theme.
"I didn't have the technique to handle the difficulties of contemporary music.
While it is undeniable that the ball is moved around the field under this form of distribution, there are inherent difficulties of technique when it comes to gripping, delivering and receiving the thing.
The cost of all this, and of the rehearsals called for by appalling difficulties of technique and co-ordination might well have dismayed the B.B.C., so that whatever anyone may have thought of the work, one can only applaud the fact that such broadcasting experiments are made from time to time.
This technique overcomes the extreme difficulties of determining effective diffusivities of the products under reaction conditions in pellet diffusion cells.
The difficulties are not merely difficulties of technique and of avoiding the pitfalls of a perfect solution impossible to administer, but are questions of principle that raise issues as to the proper locus of power over new capital investment.
Her larger crusade, though, continues here in Gaza, where she established the Community Media Center in 2007 to train Palestinians in using documentary films and other techniques to expose the difficulties of daily life.
