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Genes that were technically validated were selected for biological validation in an independent set of 58 lung samples from the TPCH tissue bank; these samples were selected on the basis of the patients' FEV1 and FVC measurements.
The GammaKey has been technically validated by standards established by the National Electrical Manufacturers Association.
To date, however, communications quality has not been technically validated in any of the various built (churches, museums, archaeological sites) or natural (caves, lava tubes) heritage scenarios.
Indeed, after nearly three decades of surgical robotics research, many prototypes have been built, some have been technically validated, but just few found their way in the OR.
Data have been technically validated on a contralateral dermal tissue from the same subject and compared with those from control healthy skin.
Genes identified were technically validated using qRT-PCR with primers designed to amplify the same transcripts represented on the microarray platform.
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Due to sample limitations, we were not able to technically validate all 21 CpG hotspots, so we randomly selected 4/21 genes for technical validation using MiSeq.
Quantitative RT-PCR was performed for selected genes to technically validate the results obtained in the microarray study.
Thus, we were able to technically validate our results with pyrosequencing, and analyze ten times as many CpGs with the BisPCR method.
To technically validate the data generated in the microarray study, quantitative RT-qPCR was carried out on selected candidate genes (Additional file 3).
In order to technically validate the results of RNA-seq, we performed qRT-PCR Eco™ Real-Time PCR system (Illumina, CA, USA).
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