ER-cap requires submucosal lifting and positioning of a snare in the cap, making it technically demanding and laborious.
MMS has advantages in controlling the tumor burden microscopically, but it is laborious, technically demanding, expensive, and time-consuming, which are major disadvantages [ 14].
The procedure of extracting TILs, priming and expansion in vitro and application to the host over longer periods is technically demanding and laborious, thus expense will be a limiting factor for the design of larger clinical trials.
Overall, this novel method was shown to be quantitatively comparable to previously used methods, but may be superior as an adequate tool for routine applications in large international field trials as it is technically less demanding and laborious than previously used methods [25].
On the other hand, NGS approaches are time-consuming, laborious and technically challenging.
However, real-time PCR is technically demanding and quite laborious and must be done in a sophisticated laboratory.
A laborious and technically difficult cell culture cytotoxicity assay is still regarded as an alternative reference standard, but is seldom performed in most microbiological laboratories.
23, 24 Despite being considered to be the gold standard for the measurement of HIV-1 coreceptor usage, the phenotypic method has some disadvantages that should be kept in mind: the method is technically complex, expensive, and laborious.
Some of these reports employed a laborious and/or technically challenging method to identify or clone only one single amplified gene.
While critics have deplored providing proprietary protection for such "products of nature," the work necessary to obtain gene and protein products having therapeutic value is admittedly laborious and often technically demanding.
