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It is technically difficult to establish TGFβ profiles in unwounded intact skin, since human skin isolated by biopsy is in fact "wounded" tissue.
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In a bioterrorist scenario it would be technically difficult to give this drug to a large number of exposed individuals.
It is technically difficult to unambiguously establish whether these synchronously dividing germ cells truly belong to a single cluster, or whether individual cyst cells within a cluster might divide asynchronously.
As a result of the speed at which the early embryo grows and develops, conditional gene deletion in the node is technically challenging, and it is difficult to establish whether all protein has been lost from a cell.
This attitude was pervasive: administrative practices remained unaltered; guilds were set at prescribed numbers, and new ones were difficult to establish; most exports were technically illegal; and estate holders were not legally entitled to bequeath their estates to their heirs.
This is difficult to establish.
Causality is always difficult to establish.
A formal mechanism proved difficult to establish.
Trends over time were difficult to establish.
That has made it difficult to establish a national figure.
If this assumption is correct, then larger-scale epigenome studies will prove very technically difficult to perform.
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