For both lines, the level of protein expression variation was in the range of technical variation.
But according to MTA spokesman Adam Lisberg, the current beeps, as unappealing as they might be, stem from "natural technical variation and we really don't care".
Thus, technical variation can substantially affect the interpretation of microarrays.
Therefore, this CV is an assessment of technical variation.
The day and process used to isolate mRNA are two additional sources of technical variation.
Technical variation between gene expression levels remained below 5% within one sample.
Standard normalization methods are designed to remove rather minor technical variation evenly distributed across samples.
Together these data suggest that good experimental practice can minimize the effect of technical variation.
The statistical analysis took account of both biological and technical variation.
So, it is not clear whether all the variation removed was technical or whether all the technical variation was removed.
Biological replicates were analyzed two or three times to determine the technical variation of MS acquisition, as discussed further below.
