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The custom assay was optimized against bisulfite sequencing as described [ 56], resulting in the conservative technical threshold of Dm ≥ 0.15 for methylation detection.
The sequencing read depth was independent of ompA serovar, but related to input genome copy number with the two vaginal swab samples that failed to generate full genomes, falling below the current technical threshold of ~552,000 genome copies per enrichment reaction.
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The Dm value of 0.15 or above (corresponding to 15%% of methylated DNA) was treated as the conservative technical threshold for methylation detection [ 49].
In brief, technical thresholds for a reliable detection of methylation were determined by evaluating MS-MLPA results against bisulfite sequencing.
The avgEL of EB1 genes was the average of the hybridization signals of experiments that showed a value of hybridization signal >75 (technical threshold).
A method technical threshold was set to 90% similarity, above which significant differentiation of the isolates was not possible.
With nuclear or biological weapons, the technical threshold is high.
Stocks could surge into the new year if the S.& P. 500 passes a couple of crucial technical thresholds, said Todd Salamone, research director at Schaeffer's Investment Research.
"In becoming a great nation, China will definitely need to leap over technical thresholds and mental thresholds.
The N.B.A. enacted the 16-technical threshold before the 2005-6 studyingseveralg several years of totals and arriving at the number as a total that warranted an additional penalty.
"It's just that the vast majority of players have been able to control their passion for the game in what's happening on the floor and are not approaching the 16-technical threshold.
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