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When we reach the computer screens showing animated pre-visualisations of complex technical sequences, Starkey talks me through a shot that Zemeckis had come up with the previous week.
The stunt that Mr. Tierney was performing was, in fact, one of the less ambitious technical sequences; it was not one of the two dozen flying scenes that raised concerns about safety this fall, for instance.
The show's maiden performances have drawn comment since the creators are still working on technical sequences; at the first preview the show had to stop five times to deal with glitches.
In this scenario, AdapterRemoval performed particularly well, reflecting its relative strength in removing technical sequences.
Trimmomatic uses two approaches to detect technical sequences within the reads.
The standard 'seed and extend' approach (Li and Homer, 2010) is used to find initial matches between the technical sequences and the reads.
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Figure 1 illustrates the alignments tested for each technical sequence.
we run the risk of incorrectly adjusting for biological sequence bias, rather than technical sequence bias.
'Simple' mode aligns each read against each technical sequence, using local alignment.
Homopolymer is a stretch of the same nucleotide sequence which has been documented to contribute to technical sequencing errors when using 454 Life Science Technology.
For the technical sequencing repeat of biological replicate #1, the library preparation and sequencing were repeated from the same viral RNA templates.
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