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Figure 1 illustrates the alignments tested for each technical sequence.
we run the risk of incorrectly adjusting for biological sequence bias, rather than technical sequence bias.
'Simple' mode aligns each read against each technical sequence, using local alignment.
The algorithmic approach used for technical sequence alignments is somewhat unusual, avoiding the precalculated indexes often used in NGS alignments (Li and Homer, 2010).
Note that the current technical sequence identification approaches in Trimmomatic are not designed to filter or categorize data on the basis of 'barcodes'barcodes
The process begins with a partial overlap of the 3′ end of the technical sequence with the 5′ end of the read, as shown in (A).
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When we reach the computer screens showing animated pre-visualisations of complex technical sequences, Starkey talks me through a shot that Zemeckis had come up with the previous week.
The stunt that Mr. Tierney was performing was, in fact, one of the less ambitious technical sequences; it was not one of the two dozen flying scenes that raised concerns about safety this fall, for instance.
The show's maiden performances have drawn comment since the creators are still working on technical sequences; at the first preview the show had to stop five times to deal with glitches.
Even in carefully executed experiments, the unmapped reads can comprise a considerable fraction of the complete set of reads produced, and can arise due to technical sequencing produced by low-quality and error-prone copies of the nascent RNA sequence being sampled.
In this scenario, AdapterRemoval performed particularly well, reflecting its relative strength in removing technical sequences.
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