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Applying all ECMs as a package gives a potential technical reduction in final energy demand of 55% and a 65% reduction in CO2 emissions, while the corresponding techno-economical potentials are 33% and 37%.
In addition, we devised an innovative new class of markers called "haploSNPs" as the basis for achieving a technical reduction in ploidy and thereby diminishing the problem of cluster compression associated with SNP array genotype calling in polyploids.
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In addition to "technical ploidy reduction", our outcome benefitted from "biological" ploidy reduction, which derived from the fortuitous existence of localized regions of effectively reduced ploidy in an otherwise octoploid genomic context, with the ploidy reduction assumed to be the result of large or small scale insertions and deletions among subgenomes.
In contrast, diploid clustering for the SNP-SNPs was more prevalent in the zones of higher read depth, and particularly at the presumed octoploid level, indicating that the diploid clustering resulted from the effectiveness of the technical ploidy reduction strategy and not from underlying biological ploidy reduction.
Therefore, in the following discussion of various technical ploidy reduction strategies, the possibility must be recognized that SNP sites targeted for technical ploidy reduction strategies might also correspond to sites of biological ploidy reduction.
We also look for its ability to garner significant value increase via technical risk reduction and/or opportunity elucidation within one two years, allowing for a fund-raising event via strategic or investment capital that would continue development.
Technical ploidy reduction was sought by means of four SNP discovery strategies.
We exploited data set A as a valuable resource for analyzing the technical variability reduction along the normalization steps.
In the design of the array, strategies were successfully developed and applied that enhanced cluster resolution by achieving technical ploidy reduction.
In order to analyze the pattern of technical variability reduction, we took advantage of data set A, which showed a large level of biological and technical replication and contrasted conditions with expected consistent differences in methylation.
In the present study, we devised and successfully implemented strategies for "technical" ploidy reduction, in which three categories of haploSNP sites were identified and targeted with probes intended to have subgenomic specificity.
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