Table 13 Adapted agile practices Category Practice Companies Adaptation Technical Pair programming A, B, C, D, E, G Employ when necessary.
Dye-swap technical replicate pairs were included for each of the biological replicates.
This dataset was used to assess the performance of normalizations and EB batch correction by comparing the 9 technical replicate pairs.
Actually, the impact on the variability reduction was minor after SWAN and unfavorable after SQN or GS, when evaluating the discrepancy between technical replicate pairs (Fig. 2, 4 and 6; Figs. S7 and S8 ).
The performance of each normalization was evaluated by comparing the errors of all CpGs between 9 technical replicate pairs, which were obtained by calculating the differences for each of CpGs between two replicates and they should be zeros theoretically.
Markers were filtered on the basis of reproducibility (that is, the percentage of technical replicate pairs scoring identically for a given marker), call rate (that is, the percentage of samples for which a given marker was scored), and the average read depth (that is, the average number of sequence 'tag' counts contributing to the genotype calls for a given marker).
One subject had one technical replicate pair per condition, with one of the two members of the pair for each batch.
"The Maltese Falcon was an intellectual endeavor," Mr. Coste said, "exceptional because of its modern technical features paired with a vintage look".
A total of 12 matched samples, including one technical replicate pair (same DNA hybridised onto different arrays on same slide), were selected.
The level of discordance in technical replicates, twin pairs, and unrelated pairs was compared by using the mean of the ED in methylation within technical replicates of six samples, three randomly selected BMI concordant twin pairs and six randomly selected unrelated, same-sex individuals.
