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Samples were designated as positive if the LSI was greater than or equal to 5. Assays were considered invalid if there was documentation of technical failure due to lack of tritium incorporation, high background cpm, not enough cells to complete the assay or lack of response to the mitogens.
Unfortunately, 5/6 nephrectomy itself in rats caused 20% of drop-out, with even higher drop-out when technical failure due to omentum wrapping of the catheter during the PD treatment was taken into account.
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Assays exhibiting poor genotype clustering after two rounds of optimization were considered technical failures due to quality control violations [34].
Second, the possible use of low amounts of DNA from any kind of (uncultured) tissue diminishes the technical failures due to the in-vitro cell culturing process [29].
Three cases were technical failures due to insufficient tumor tissue.
Four TS procedures ended in technical failures due to pleural adhesions.
Results: two immediate technical failures occurred due to malposition.
Particularly in the early phase of the study, we had several technical failures mainly due to the use of ComfortScan hardware, which is an evolving digital optical scanner.
Some of the failures to amplify junctions and fusion transcripts may have been technical failures, or due to errors in mapping the paired sequences, or because the rearrangements were more complex than the automated analysis revealed.
And at the end of the year, Air Asia flight 8501, an Airbus A-320 aircraft with 162 people onboard, crashed apparently because of technical engine failures due to weather.
"There is no technical failure, it is simply due to demand".
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com