The problem responsible for Tuesday's scrub decision related to a technical issue detected in the steering mechanism of the rocket's upper stage.
We attribute the lack of detectable binding at most Class II sites to the speed with which Pif1 family helicases unwind G4 structures [ 19], the stringent criteria used to identify binding sites, and technical difficulties detecting DNA helicases by ChIP [ 18].
Sequencing of the dihydrofolate reductase (pfdhfr) and dihydropoteroate synthase (pfdhps) genes (Technical Appendix) detected 4 SNPs in pfdhfr associated with pyrimethamine resistance (N51I, C59R, S108N, and I164L) and 4 SNPs in pfdhps associated with sulfadoxine resistance (S436A, A437G, K540E/N, and A581G) (Table 1).
The analysis presented here is developed for data from two-hybrid screens alone and only detects technical false positives.
In our study, there were no technical problems in detecting mutations; the frequent detection of SNPs demonstrates the high sensitivity of our procedure.
Again there is a great possibility of utilizing light weight UAS in PV plants monitoring due to various advantages such as high flexibility, large area coverage, precise imagery, cost efficiency, fast detection, ability to operate in hostile environments, high technical availability to detect defects by accurate sensors, and special cameras like infrared and thermal models [12, 13].
The fact that both GFP::SAS-4 and SAS-6::GFP were no longer detected in the majority of cases towards the end of embryogenesis could reflect technical limitations in detecting centriolar proteins by immunofluorescence at late stages.
The analytical sensitivity and specificity to detect a KRAS mutation in a clinical CRC sample will depend on (i) the technical ability to detect mutated alleles in a background of normal wt alleles, (ii) the sensitivity to DNA degradation in terms of DNA template target lengths, and (iii) the defined thresholds and signal strengths required for a positive or negative mutation call.
The conflicting observations may be the result of technical difficulties in detecting IL-17 in paraffin-embedded synovial tissue, the use of polyclonal antibodies to detect IL-17, and clinical differences between patients studied.
This means better technical systems to detect what people will flag as false before they do it themselves".
