These differences make it difficult to compare results from screening experiments and laboratory and technical scale cultivations and, thus, complicate rational process development.
So far, there has been limited interest in tackling the biological and technical feasibility of cultivation of C. concholepas.
Many medicinal plants, however, are still harvested in the wild due to technical difficulties of cultivation, as well as for economic reasons.
We thank Ashley Hayes for providing the different cell lines, Veronique Krumb for technical assistance concerning cultivation and transfection of cells, Marc Bedoucha and Olivier Partouche for the cell sorting, Doris Zulauf for technical assistance concerning hybridoma generation and Bernard Rutten for Ab purification.
Meanwhile, with the responsibility for knowledge transformation, sci-tech talents cultivation and technical innovation, universities are of great importance in the development of the sustainable society.
for technical support in hop cultivation.
Three clones were classified as holoclones, forty-two as meroclones and three as paraclones; four clones were lost during cultivation for technical problems (Supplementary Table S1).
Thus, the present study is aimed at determining the effect of carbon to nitrogen ratio and organic nitrogen source on the cell growth and P 3HB- co-3HV) production by Comamonas sp. EB172 in 2 L fermenter by a pH-stat fed-batch cultivation using technical grade VFAs.
Technical scale (≥5 l) cultivations of shear stress sensitive microorganisms are often difficult to perform, as common bioreactors are usually designed to maximize the oxygen input into the culture medium.
To test the technical feasibility of A. bisporus cultivation on self-heated pasteurized substrates, three substrates without supplements were assayed initially.
The project offered the producers technical support to improve their cultivation of alternative sources of income--such as cacao, coffee, honey, and African palm--but more importantly, helped the producers build new marketing channels to sell the alternative products.
