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Following approval by the group, this document was reviewed and approved during a cabinet meeting before distribution as a technical cluster note to all WHO members.
Drawing insight from the theoretical lens of Sociotechnical theory, the seven clusters of factors required for HRA implementation could be read as belonging to three overarching aspects : Technical (cluster 1, 2 and 3), Social-Patient (cluster 4 and 5), and Social-Provider (cluster 6 and 7).
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Clustering analysis showed that major differences exist between the two biological groups and that technical replicates cluster together even before any normalization, as evident from multidimensional and hierarchical clustering (Fig. S1A and B).
All technical replicates clustered adjacent to each other, or with only a single sample separating them.
Four out of five technical replicates clustered either immediately adjacent to one another or with a single sample separating them (black bars, Figure 1A).
The two technical replicates clustered together on the dendogram.
Moreover, because different ChIP-seq experiments have distinct technical specifications, clustering directly on raw counts tends to be influenced more by the ones with higher signal to noise ratio.
A pipeline was positively scored whether (1) technical replicates clustered together, (2) the correlation increased while (3) the average absolute difference between technical replicates decreased and (4) the bias between Infinium probe types decreased as well (for a detailed description see Materials and Methods section).
Performing unsupervised hierarchical clustering, all of the technical replicate sets cluster with each other.
"The addition of VMWare's technical expertise in cluster infrastructure will enable people begin to compute like Google, regardless of where they physically do that computation," says Google product manager Craig Mcluckie.
We identified 5 clusters during the first wave (February April 2013) and 8 clusters during the second wave (November 2013 May 2014); the clusters in both waves had median size of 2 cases per cluster (Technical Appendix Table 2).
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