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Data on health care use, patient characteristics, and pathology information of tiotropium users was drawn from two existing data sources: the IMA Common Sickness Funds Agencyy) claims database and the MCD-HBD (Minimal Clinical Data - Hospathologyling Dand) pathology and claims database of in hospital stays from the TCT (Technical Cell).
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This study aims to demonstrate the technical and cell biological feasibility of using scaffold-based tissue engineering in ACO.
It is also important that for the successful implementation of cellular manufacturing, that one focuses both on technical issues (cell formation and design) and human issues.
Scatter plots and the corresponding MVA plots are shown in Figure 2 for three selected technical replicates (cell lines) from SAM 1.
Correlation functions from these traces reflect technical bias (cell movement, coat protein diffusion, imaging or tracking artifacts) and are mostly flat.
Even the most trivial deviation from a technical protocol culturing cells, quantifying proteins, detecting RNA can make an experiment difficult to replicate.
As both datasets were collected using ChIP-Seq, this sharp divergence is more likely biological than technical: PC3 cells do not express androgen receptor endogenously and its AR binding was profiled following transfection of an AR construct [ 11].
Until now, one of the technical limitations of cell death studies came from the fact that myofiber cell death is always increased in the muscles of muscular dystrophy subjects (see Figures 7 abc&d).
When combined with new technical methods in cell processing and culturing, the therapeutic potential of T cells manufactured in academic centers has improved dramatically.
Other technical challenges for cell manufacture include the requirement to use proteolytic enzymes to detach cells from support materials during passaging or after cell expansion.
Although the level of circulating tumour cells (CTCs) is prognostic and associated with chemoresponse in CRPC, quantitation is cumbersome and prone to technical challenges including cell lysis (Danila et al, 2007a; de Bono et al, 2008; Scher et al, 2009a).
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