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Next, the ComBat algorithm was used to estimate and remove the technical bias (array type, scanner and vehicle) from the normalized data matrix [35].
Apart from the bias linked to who is using Twitter, there is also a technical bias imposed by how the data is collected.
However, the actual similarity between Nipponbare and CG14 at polymorphic sites might be closer because a technical bias would exist when the sequence reads of the parental lines were aligned upon the Nipponbare genome sequence.
The system did not reveal a major technical bias for low CS concentrations, up to 20%, which however correspond to a concentration range high enough to cause a decrease in cell viability in two different cell lines tested.
that increase the chance of technical bias.
Randomization of measurement order and normalization of the data will eliminate the technical bias.
These studies deploy different types of microarrays on species with varying levels of divergence, and these experimental variables influence the potential for technical bias.
All samples were randomized in the experiment and each sample was independently processed in triplicate for the entire experiment to control for potential technical bias.
Furthermore, whole blood cells are easy to collect and store during field studies on large cohorts, reducing samples volumes required and limiting technical bias due to cell purification.
RMA method was applied to the primary data to correct the technical bias and summarize gene expression values over probe-sets [36].
Before the methylation data were analyzed, 84 CpGs located on chromosome X and 11 CpGs showing interlab differential methylation (i.e. interarray version) were excluded to avoid possible sources of biological and technical bias.
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CEO of Professional Science Editing for Scientists @ prosciediting.com