Such extreme observations can be due to the presence of very short segments of DNA with deviant copy numbers, to technical aberrations, or a combination.
We can surpass our current technical limitations by correcting the aberrations of the 4 π-PM.
Technical comments: 1) The accuracy of correction of chromatic aberration was not estimated, but it is expected to be in the range of measurable values.
Somewhat surprisingly, they failed to qualify for the women's team sprint and it was this aberration that indirectly led to the resignation of the technical director, Shane Sutton, paving the way for Harrison to take over.
This technical limitation does not invalidate our finding that Y-iPSCs aberrations are mainly DNA demethylation failure.
This technical approach is also evidently able to reveal copy number aberrations, as we encountered a 47, XXY neonate in our sample cohort.
Multiplex ligation-dependent probe amplification is a PCR-based technique that detects a large variety of segmental aberrations and gene amplifications in a robust manner (for technical details, see (Schouten et al, 2002).
Since our cohort of ALL patients included samples diagnosed as early as 1996, the fact that the canonical aberrations were not detected at diagnosis could be due to technical limitations of the methods applied at that time.
Direct identification of mutations by sequencing has the advantage of pinpointing the specific aberration, but is not currently applicable to clinical practice because of its expense, technical difficulty, and likely reduced sensitivity in the routine setting.
Integrated approaches that not only account for DNA and RNA aberrations but also document protein function and biochemistry are clearly the next technical horizon [ 60].
