TC: Acting is something that we all practice at some time in our lives.
Additionally, TC acts as a final screening mechanism because, like IF, TC is very specific for cobalamin forms that have the lower DMB intact (Refs 73, 74).
This slippery term refers to the fact that TCs sometimes act to define the academic territory of a discipline.
However, the TC-induced inhibition was partial (∼30%) compared to the effect of carbachol (Fig. 3b, c), suggesting that TC acts as a partial agonist of this receptor.
To detect the In 3R) Kodani inversion, we used the following primers normally located on the same DNA strand: In 3R K-R1/F1 (5 ′ -TCG AAG CCC GTG TGG TAA TC), which acts as forward primer if inversion present, and In 3R K-R3 (5 ′ -TTC TCC CAA CGC ATC ACC AAA).
The C291A and C332A mutants of SmARG were prepared by PCR mutagenesis with the following primers (underlined bases indicate mutated codons): C291A, 5′-GAA GGT TTG AGA ATA GCT GAA GAA GTT TC-3′ (sense) and 5′-GAA ACT TCT TC A GCT ATT CTC AAA CCT TC-3′ (antisense); C332A, 5′-CAT ATT TTA AGA GCA GCT TTA GGC CAT TGT CG-3′ (sense) and 5′-CGA CAA TGG CCT AA A GCT GCT CTT AAA ATA TG-3′ (antisense).
Approximately 1-kb DNA fragment upstream of the start site of egl-15 gene was amplified from worm genomic DNA by PCR using the following oligonucleotides: 5′-ACA TGC ATG CAA TCG CAC AGA TTA AAT TAC ACT TC-3′, and 5′-TTG GCG CGC CGA TGA TAT GTA TCA GGC AAA CAT TC-3′.
Before the mid-1990s, the PTCe was small, and TC did not act as the primary trigger for monsoon onset.
Following nucleic acid purification, the 5' part of the 16S rRNA gene (corresponding to Escherichia coli positions 10 to 806) was amplified using primers V1 [5'-AGA GTT TGA TCA TGG CTC AGA] and V3 [5'-GGT TAC CTT GTT ACG ACT TC].
Primers for human β-actin were 5′-AAA CTA CCT TCA ACT CCA TC-3′ (forward) and 5′-ATG ATC TTG ATC TTC ATT GT-3′ (reverse).
