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The significance of using the TCR in the numerical model is investigated by comparing constant and variable TCR (i.e. position dependent) at the interface.
Resistivity values for the cermet films (55±10 vol.% Ni) were in the range of 2.5×10−4 6.5×10−2 Ω cm, with linear temperature coefficient of resistance (TCR), i.e. α of up to 4200 ppm/°C.
The positive selection and differentiation in the SP cell occurs when the DP cell TCR(i) receptor interacts with an intermediate affinity.
If ΔN i) is larger than θ2, a low affinity exists between the DP cell TCR(i) receptor and the MHC/peptide of the epithelial cell, also resulting in the thymocyte death by apoptosis.
If ΔN i) is smaller than θ1, high affinity exists between the DP cell TCR(i) receptor and the MHC/peptide of the corresponding epithelial cell, resulting in thymocyte death by apoptosis.
For a given population repertoire the Kullback-Leibler distance against the superpopulation was calculated as the sum of the products between pk and log pk/qk) where pk denotes the population frequency of the k-th TCR and qk denotes the pooled frequency of the k-th TCR (i.e., frequency in the superpopulation).
Similar(53)
T-cell receptor (TCR) engagement, i.e. activation of T lymphocytes leads to the expression of Fas (CD95) on the surface of the activated T-cell, a prerequisite to undergo activation induced cell death (AICD) [49], [50].
Once we carefully selected putative positive clones for TCR probes (i.e. only selected the strongest signals among the neighboring signals), the percentage of true positives increased (see Table 2, 89 91% TCRs).
As a result, many of the readouts downstream of TCR activation (i.e., CD69, CD25, ppERK see Figure 1A) are all-or-none, and gradations in signal strength are resolved only by counting the number of activated T cells.
To further evaluate the reliability of homologous peptide antigen derived from the iMatrix, we collected the 80,057 experimental peptides from the IEDB (January 2013) for 389 pathogens; and 17 TCR-pMHC complexes (i.e. TCR-peptide-HLA-A0201, Table S4 in Additional file 6) from the PDB.
To test the relative affinity of the two TCRs for I-Au Ac1-11 complex, we fI-Au Ac1-11ted purified T complexith different doses of F23.1 [30], an anti-Vβ8.2 mAb that we have previously shown does not cause activation ofirstells at the concentratincubated for these expurifieds [21].
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