Sentence examples for tcc a from inspiring English sources

For the unmethylated condition (U), a sense primer 5' GTT TGA GGT TGT ATT GGG TA and an anti-sense primer 5' ACT CAC AAC TCC AAC TCC A were used.

The primers (Sigma-Aldrich, Germany) used for the coamplification of PVL genes lukS-PV and lukF-PV were lukPV-1 (5′-ATC AGG AGG TAA AAT GTC TGG ACA TGA TCC A) and luk-PV-2 (5′-GCA TCACTCT GTA TTG GAT AGC AAA AG C [ 26].

The following primer pairs were used to amplify the mRFP1 region of the target RNA: Forward: 5' TAC GAC GCC GAG GTC AAG 3' Reverse: 5' TTG TGG GAG GTG ATG TCC A 3' and for 16S rRNA: Forward: 5'CGT CAG CTC GTG TTG TGA A 3' Reverse: 5' GGA CCG CTG GCA ACA AAG 3' For details and results of qPCR measurements see Additional File 2. We detect cells from raw images as in [ 32].

Primers were designed to amplify across an exon/exon boundary (leptin forward: ACC AAA ACC CTC ATC AAG ACA ATT, leptin reverse: TCC AAA CCG GTG ACT TTC TGT T, leptin probe: ATT TCA CAC ACG CAG TCA GTC TCC TCC A, CD45 forward: CGT AAT GGA AGT GCT GCA ATG T, CD45 reverse: CTG GGA GGC CTA CAC TTG ACA, CD45 probe: ACA ACT AAA AGT GCT CCT CCA AGC CAG GTC T).

For the TCC, a confirmatory test by transferring 1 ml of the aliquots from each dilution into three tubes of Lauryl sulfate tryptose (LST) broth and incubating at 35 °C for 48 h was also done.

Table 1 Nucleotide sequences of RAGE and BAC primers and probes Primer/probe Nucleotide sequence (5′ → 3′) RAGE forward primer CCC TGA CCT GTG CCA TCT CT RAGE reverse primer GGG TGT GCC ATC TTT TAT CCA RAGE probe [6FAM] CCC AGC CTC CCC CTC AAA TCC A [BHQ1] BAC forward primer GTA TGG GTC AGA AGG ACT CC BAC reverse primer GTT CAA TGG GGT ACT TCA GG BAC probe [TET] CCT CTC TTG CTC TGG GC [BHQ1].

Primers targeting the glycoside hydrolase families 5 (cel5 _392F: 3′-GAG CAT GGG CTG GAA YHT NGG NAA-5′ and cel5 _754R: 3′-CAT CAATAAT CTT TGA AGT GGT TTG CAA TYT GDK TCC A-5′) and 48 (cel48 _490F: 3′-TNA TTG TTG AAG CTC CDG AYT AYG G-5′ and cel48 _920R: 3′-CCA AAN CCR TAC CAG TTR TCA ACR TC-5′) were used to study the cellulose-degrading bacterial community structure [24].

Similarly, for mouse β-actin: forward, 5'-tgg aat cct gtg gca tcc a-3' and reverse, 5'-taa cag tcc gcc tag aag ca-3' primers were used.

Hydroxymethylbilane synthase (Hmbs, F: 5'-tcc ctg aag gat gtg cct ac-3' and B: 5'- aca agg gtt ttc ccg ttt g- 3') and aminolevulinate synthase (ALAS, F:5'- cca tca att acc caa cag tgc-3' and B: 5'-gtg acc agc agc ttc tcc a-3') were used as housekeeping genes to normalize quantification data.

The FLT3 mRNA transcripts were quantified by quantitative real-time reverse transcriptase-PCR, using forward primer 5′-CAG GAC TTG GAC AGA GAT TTT CAA-3′, reverse primer 5′-TCC GGG TGT ATC TGA′ACT TCT CTT-3′ and probe 5′-FAM-CCC ACT TTC CAA TCA CAT CCA AAT TCC A-TAMRA-3′.

Two primers with the sequences, TR-8 (5′-TGC ACG TCG CGG ACC TCC A-3′) and TR-9 (5′-TCG CGA CGA TCA AGG AGT-3′) were used to amplify a 187-bp fragment of the RRDR in rpoB gene.