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Concerning the process using anionic PSt particles after surface modification by KH550, 5 mL of anionic PSt latex (of 9.40 wt.% solids) and 0.10 g of KH550 were first added into 50 mL of EtOH; the mixture was stirred for a few minutes and followed immediately by addition of a TBT (1 mL) solution in EtOH (10 mL) within 10 min at a constant rate under vigorous agitation.
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As the extended authorisation requirement features all characteristics of a technical regulation set out in No. 1 of Annex 1 to the agreement on technical barriers to trade (TBT) the scheme is subject to the legal assessment criteria provided in TBT [15].
As expected, fish exposed to TBT showed a bias of sex toward males (62.5% males in control tanks and 86% and 82% in TBT 25 and TBT 100 ng TBT/g, respectively).
Co-exposure to EE2 completely blocked the masculinizing effect of TBT, with 7% males in the TBT 25 ng/g + EE2 treatment and 0% in the EE2 alone and in the TBT 100 ng/g + EE2 exposed groups.
The mean time was 5.36 min in TBT, 22.6 min in TBP, and 59 min in TLS.
Hence, EG and HAc were introduced into the mixture to co-control the hydrolysis rate of TBT [29].
Two months exposure to TBT (5 and 50 ng TBT L−1) induced imposex and led to a significant increase in the severity of the phenomenon in the high TBT exposure group but statistical tests failed to detect an effect in 5 ng TBT L−1 treatment.
Meanwhile, a titanium precursor composed of 1.5 mL TBT, 7.21 mL EG, and 1.14 mL HAc was also chilled to ≈ 5°C in an ice bath.
In females of B. brandaris, 2 months exposure to TBT at 50 ng TBT L−1 induced a significant elevation of free testosterone and a decrease in the esterified fraction.
Three hundred sixty healthy zebrafish were randomly grouped into four groups and exposed to different doses of TBT (0, 1, 10 and 100 ng L−1).
The activity and expression of lysozyme and the content of immunoglobulin M were significantly decreased compared to those of the fish exposed to 0 ng L−1 TBT (p<0.05).
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