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Thereafter cells were treated with 400 ng/mL Gas6 or TBS control and incubation was continued for another 24 h.
We could not detect any effect of Gas6 stimulation on the invasive phenotype of 786-O cells in comparison to TBS control (Fig. 4B).
Gas6 stimulated cells showed phosphorylated Axl, while TBS control treated cells show no or markedly low basal Axl phosphorylation levels (Fig. 2C,D).
A modest but clear decrease in viability was seen after 24 h of Gas6 stimulation (400 ng/mL) compared to TBS control stimulated cells (Fig. 4A).
Simultaneously, 400 ng/mL Gas6 (final concentration) or TBS control were added to both the upper and lower chamber and the cells were allowed under 16 h at 37°C to invade the matrigel and migrate.
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Bovine TB control currently costs the government (in other words taxpayers) about £90m a year.
The United States recently doubled foreign aid grants for TB control, to $22 million.
I put that question to Mannan, the official responsible for TB control in the Bihar region.
Killing badgers isn't the long-term or sustainable solution to bovine TB control that farmers so desperately need.
In Connecticut, TB cases have been on the decline since that time, said George Raiselis, acting director of TB control for the State Department of Public Health.
But ministers have insisted the cull is a necessary part of bovine TB control measures which cost taxpayers £100m a year for TB testing and farmer compensation.
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