Sentence examples for tbs 1 from inspiring English sources

Then, the slides were rinsed in Tris-buffered saline (TBS) (pH 7.6) and incubated with normal swine serum (NSS) in TBS (1 : 5) to block nonspecific staining.

The strips were blocked with TBS, 1% western blotting reagent (Roche) at 4°C, O.N.

Phospho p44/p42 MAPK staining was essentially carried out as described for c-MYC with the following changes: TBS, 1% Tween 20 (TBST) was used for washes.

Cells were lysed in 100 µl Tris-buffered saline (TBS), 1% Triton X-100 1x EDTA-free complete protease inhibitor cocktail (Roche).

Control and mutant cerebellum were dissected from age matched P3, P7 and P11 mice and lysed in RIPA buffer (1X Tris buffered saline (TBS), 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 0.04% sodium azide, 1 mM PMSF).

Coverslips were then rinsed three times in TBS (1 M tris-HCl, 1.5 M NaCl, pH 7.5) and 100 µl of blocking buffer [(1% (v/v) FBS, 1% BSA (w/v) in TBS] was added.

made up in TBS (1 30) containing 2 % BSA overnight at 6 °C.

All serum samples were diluted 1 5 with sample diluent (TBS +1% Bovine serum albumin +0.05% Tween 20).

Nonspecific binding of primary antibody was blocked by using normal swine serum (NSS, in Tris-buffered saline (TBS) (1 5), 100 μl/slide) for 10 minutes of incubation.

Samples were incubated with the primary mouse anti CD99 antibody (diluted at 1 50) at room temperature and rinsed in TBS 1× pH = 7.6 Tween 0.05%.

Secondary biotinylated goat anti mouse antibody (Dako, E0433) diluted at 1 200 was applied 30 min at 37°C and rinsed in TBS 1× pH = 7.6 Tween 0.05%.