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These experiments proved a key role for the CD8+ T-cells in the pathology of TBE, and that TBE is essentially an immunopathological disease [ 15].
Moreover, there is no clear internationally standardized case definition of TBE, and apart from Austria, no clear perception of TBE risk.
Place the gel into the running chamber containing 0.5× TBE and remove the combs.
Total PCR products were electrophoresed on 1.25% agarose gel in TBE and stained with ethiduim bromide.
The mixture was then loaded onto a 6% DNA retardation gel in 0.5X TBE and run at 100 V for 1 h at 4 °C.
cDNA was then gel purified by denaturing 10% PAGE (7 M urea, 1X TBE), and extended products were excised from the gel and eluted overnight at room temperature in 200 mM KCl, 50 mM KOAc.
Reactions were quenched by the addition of 2 × loading buffer (8 M urea, 0.5% TBE) and 4 μl run on an 18% PAGE at 350 V for 8 h, which was then visualized by phosphorimaging using a Typhoon Scanner (GE Healthcare).
A larger proportion of the SOC is associated with microaggregates and silt plus clay fractions in soils affected by clay illuviation (namely: Typical Luvisols (TLu), Stagnic Luvisols (SLu) and Typical Surface-water Gleys (TSWG)) than in Brown Earths (Humic Brown Earths (HBE), Typical Brown Earths (TBE) and Stagnic Brown Earths (SBE)).
The metrics considered are the number of error packets, the TBE, and the number of request packets.
Therefore, only data for the radius of 500 μm with 1× Tris-borate-EDTA (TBE) and 80 cP at Re = 5 × 10−4 (Wi = 12.5) was presented, as shown in Figure 4.
Gel electrophoresis was performed with 1% (w/v) agarose in TBE and visualised by UV transillumination after staining with Redsafe Nucleic Acid Staining Solution (iNtRON Biotechnology, Seongnam, South Korea).
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