One trained and one untrained TBA was selected from the few TBAs available in each community.
Grandmothers and TBAs were predominantly older and illiterate, whereas fathers were selected from the same households as the mothers.
They were selected because of their experiences of either being TBAs or being post-partum mothers and having received care from the TBAs.
To enhance recall, mothers, grandmothers and TBAs who had been involved in a delivery in the past three months were selected.
However, four TBAs and two community representatives who did not know how read and write were selected due to their popularity and acceptance in the community.
Electroporants were selected on TBA+Kan (20 µg/ml) plates at 37°C.
CHWs who were selected by their communities with district-led training and supervision were able to identify and visit almost all the pregnant women, especially those from the poorest families and those who delivered at home or with TBAs.
The women were selected according to the following two criteria: (i) women who had delivered in a health facility, and (ii) women who delivered with the support of a TBA.
A WATERS C18 reversed-phase HPLC column was selected, and KH2PO4 solution (200 mM), 10% acetonitrile, and TBA solution (3 mM) were used to prepare the mobile phase, and the pH was adjusted to 6.5 or 6.8, respectively.
To limit the amount of H2O present, [18F]NaF/15C5 was selected as the fluoride source to avoid the hygroscopic tetra n-butylammonium (TBA) salts.
