Exact(12)
The curated alignments were concatenated to form a 16-protein, 733 taxa alignment with 3,082 unambiguously aligned positions.
Ambiguously aligned hypervariable loop regions within the 870 taxa alignment were identified by eye and coded as binary absence (gap) and present (any base) data [ 64] before fed into RAxML version 7.2.2 [ 65].
The 5.8S re-included ITS alignment featured 1,544 positions for 296 taxa (Alignment S5).
For the second approach, SATé returned a best-scored ITS alignment of 1,296 positions for 300 taxa (Alignment S2).
We then performed additional GTR analyses under the ML framework on the reduced, 103 taxa alignment.
The species chosen for phylogenetic inferences aimed to represent the molecular diversity observed in the 870 taxa alignment.
Similar(48)
When considering transcriptome sequences from all Cornales taxa, alignments showed 26.7-62.326.7-62.3%e sites (Fig. 2).
The average taxon coverage for weighted majority was 98.6, 98.6, and 98.0percentnt for the 20,000, 40,000, and 78,132 taxa alignments, respectively.
Figure 5 shows the predicted structure of the 4-taxa alignment using our Stochastic Topology HMM (STHMM).
The AMBN sequence of the caiman (Caiman crocodilus) was added to the 53-taxa alignment using MAFFT in order to serve as outgroup.
To examine the position of Proteaceae within Angiospermae, genes were extracted from the Macadamia cp genome and added to the 83-gene, 86-taxa alignment of Moore et al. [ 4] in Geneious Pro, version 7.1.5 (Biomatters Ltd., Auckland, New Zealand).
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