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Initial (d 0) and final (d 41) body mass (BM) and total adipose tissue (TAT) mass, determined with microCT, of chow- and Ensure-fed intact rats (CH-Intact and EN-Intact), ovariectomized rats (CH-OVX and EN-OVX), and estradiol-treated ovariectomized rats (CH-OVX+E2 and EN-OVX+E2), means ± SEM.
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SAT, IAAT and TAT masses were measured periodically by microCT (LCT 100, Aloka, Tokyo, Japan).
These data were converted to masses and extrapolated to whole-body SAT, IAAT and TAT masses as previously described and validated for male rats [ 49, 50].
Rats were assigned to one of 6 groups (n = 7 each), roughly matched for body and TAT masses on the basis of d 0 data.
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Body mass, TAT, total fat mass, fat-free body mass, and %IAAT all increased in chow-fed intact rats during the 41 d study.
In chow-fed rats, ovariectomy increased excess body mass, TAT, fat mass, fat-free body mass, and SAT, but had little effect on IAAT, in chow-fed rats, leading to a decrease in %IAAT.
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