Symptoms (side effects), including headache, bloating, dry mouth, excessive thirst, fatigue or low energy, lightheadedness, nausea and change in taste, were collected via self-administered questionnaires (see online supplement) completed during the last 7 days of each sodium feeding period.
Online survey data on reading tastes were collected as previously described (Mascarenhas et al. 2007; Mascarenhas and Singh 2012).
Therefore, GAT4 was not detectable in our non-lingual gustatory epithelium sample because there were no taste buds near the area where our epithelial sample was collected.
Mouse taste bud images were collected using an LSM-710 confocal microscope (Carl Zeiss MicroImaging, Thornwood, NY) in single planes.
A simple taste test will be used to assess taster/non-taster phenotype status; a salivary DNA sample will also be collected to determine taste receptor genotype(s).
Human CV taste buds and surrounding lingual epithelium were collected by laser capture microdissection (Fig. 10A C) and semi-quantitative PCR was performed to determine the expression level of the identified genes.
In order to identify which of the receptor genes identified in the pig taste repertoire are expressed, tongue tissues were collected from 6 newly weaned piglets (24 ± 3 days of age and 9.367 ± 2.7 kg of body weight) following exsanguination (animal ethics approval: CNFS/217/11/PORK CRC).
Taste cells representing the three functional classes were collected as follows.
Occasionally, however, mushrooms of the species have been collected which have a mild taste; this has also been observed in other species with typically acrid tastes.
Data were collected on dietary intake, cognitive performance, taste acuity, haemoglobin, erythrocyte zinc and plasma levels of zinc, vitamin C, β-carotene and retinol.
