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All subjects performed the task under perfusion of ketamine (intravenous bolus of 0.12 mg followed by a perfusion of 0.5 mg/kg over 60 mn) or placebo (saline).
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Under perfusion both inhibitory and, probably more important, stimulatory signals may have been washed out of the scaffolds.
Cell proliferation was better under perfusion culture than under static culture.
We addressed engineering of TLS by culturing cancer cells in porous scaffolds under perfusion flow.
Cells remain viable inside centimeter-sized constructs when cultured under perfusion.
In this work, tubular SIS was produced and decellularized by the conventional peracetic acid agitation method, peracetic acid under perfusion along with two commonly used detergent perfusion protocols.
The HSPC/MSC co-cultures were either maintained under conventional cell culture conditions in a standard 2D well plate or in the 3D scaffold under perfusion (3D dynamic) or without perfusion (3D static) in the presence or absence of 5-fluorouracil.
The system was maintained under perfusion for 20 minutes.
This included maternal under perfusion, infarction, thrombus, retroplacental haematoma and abruption.
The children performed the task under three different conditions, matched for length of time on task.
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CEO of Professional Science Editing for Scientists @ prosciediting.com