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We searched against TAS database and predicted Ta-siRNAs in C. ensifolium.
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The current TA database contains transcripts from 82 different species (Table 2) representing 811 Mb of total sequence.
All of these had corresponding tentative contig sequences present in the soybean TIGR transcript assembly (TA) database.
In a first step to know the origin and evolution of cystatins and papain and legumain-like cysteine proteinases in plants, an extensive search of the TIGR plant transcript assemblies (TA) database was made.
Accession numbers are in Tables I, III and IV from additional file 3. C13, cysteineeine proteinases and cystatin sequences were used to search the TIGR plant transcript assemblies (TA) database [ 69].
Briefly, the sequences of Saccharomyces cerevisiae and Arabidopsis thaliana genes involved in double strand break (DSB) formation, recombination, synaptonemal complex assembly, chromosome pairing and DNA mismatch repair were used as queries for TBLASTX analysis against all green plants at The Institute for Genomic Research's (TIGR Plant Transcript Assemblyy (TA) database.
Her ta-tas shakin'.'".
Here, we systematically annotated protease genes in Tl, Tv, Tv, and Ta genomes using MEROPS database (Rawlings et al. 2012).
Here, we carefully annotated the CAZymes in Tl as well as in Tr, Ta, and Tv using Pfam database (see Materials and Methods for detail).
The SNP frequency between JN177 and the sequences represented in the wheat EST database (JN177 vs Ta comparison) was only about one half of this level (5.77 per 1000 nt) (Table 2), demonstrating that the somatic hybridization process was effective in inducing point mutations.
A list of known TAs was extracted from the TANTIGEN database of TAs http://cvc.dfci.harvard.edu/tadb/.edu/tadb/
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