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Following the approach in [12], the CRB for 3D angles of multiple targets is calculated here to obtain the bound of angle estimation of the proposed scheme.
The protein sequence similarity between two targets is calculated by sequence alignment and is defined as S t seq (t u, t v ) = align (t u, t v ) / align (t u, t u ) align (t v, t v ), where align(t u, t v ) is the Smith-Waterman alignment score [ 15] between t u and t v.
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The contrast of the targets was calculated based on the conditions at the moment of target detection using measured target and background luminance.
The three-dimensional positions of the photo targets were calculated using and in-house code in Matlab and the image processing toolbox (Mathworks, MA).
Free energy changes between the wild type let-7a targets and edited let-7a targets were calculated by RNAeval algorithm implemented in the Vienna RNA package.
Relative expression levels of targets were calculated by the comparative cycle threshold (Ct) method outlined in user bulletin no. 2 provided by Applied Biosystems.
Relative quantification of all targets was calculated by the comparative cycle threshold method outlined by the manufacturer (User Bulletin No. 2; Applied Biosystems).
To assess the importance of physical proximity, the topological distance between Apc-Cdkn1a network nodes and the respective proteomic targets was calculated.
Relative quantification of all targets was calculated by using the comparative cycle threshold method [ 24].
However, in the optimal-energy groups of these studies, energy targets were calculated and not measured.
Enrichment of these targets was calculated using ACME with default settings and a sliding window of 750 bp [ 11].
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