Sentence examples for targets integration from inspiring English sources
The host cell-encoded lens epithelium-derived growth factor (LEDGF/p75) binds lentiviral integrase and targets integration to active transcription units.
First, a spontaneous cycloheximide resistant mutant of strain JLP18 (EAP3) was selected by plating cells on YES plates containing 10 mg/L of cycloheximide (Sigma-Aldrich Co .. EAP11 was generated by transforming EAP3 with SphI-digested pEA4, which targets integration of the S. cerevisiae URA3 gene adjacent to the his5+ locus.
LEDGINs are small-molecule inhibitors of the interactions of HIV-1 IN with LEDGF/p75, a human transcriptional coactivator that protects IN against proteasomal degradation, stabilizes IN tetramers, locks the PIC onto the chromatin, and targets integration to transcriptionally active regions in the genome.
Plasmids were digested with BstEII to target integration to LEU2, and the number of copies of MPS3 integrated was determined by Southern blotting.
HEases have been designed to target disease-associated genes, such as XPC [ 7] and RAG1 [ 8], to induce targeted integration in cell lines [ 9, 10], and for targeted mutagenesis in crop [ 11].
The usage of a simulation environment preliminary to a waveform integration paves the way to target integration.
The addition of the CBX1 chromodomain to MLV INW390A efficiently targeted integration away from gene regulatory elements.
Meanwhile, the Mosdi1 R left flank and right flank, which will facilitate targeted integration of foreign DNA into wild type Mosdi1 locus, was introduced in the vector.
Targeted integration and gene repair can be mediated by the DNA-targeting specificity inherent to a particular enzyme, or rely on user-designed specificities.
piggyBac has many desirable features, including seamless excision of transposons from the genomic DNA and the potential to target integration events to desired DNA sequences.
The dual DNA-binding protein-based targeted integration strategy developed herein would allow safer and more reliable genetic manipulations for various applications, including gene and cell therapies.
