Sentence examples for targets insertion from inspiring English sources

The vector targets insertion of the cloned gene in the pBR322 docking platform that is contained within the compatible Y. lipolytica Po1g strain's genome in a single copy.

(A and B) A scheme for CRISPR/Cas9-mediated targeted insertion, which is similar to Fig. 1A; Cre-mediated reversion of targeted insertion is as follows: targeted insertion could be deleted with Cre recombinase delivery.

To enhance targeted insertion, homologous recombination was stimulated using targeted cutting at the OCT4 STOP codon with CRISPR/SpCas9.

Nested PCR assay revealed successfully targeted insertion of DOI in all the 8 blastocysts with targeted insertion efficiency of 21.6% injected zygotes that developed into blastocysts (3.9% of total injected zygotes).

As targeted insertion near transcriptional termination sites stimulated lncRNA expression, we wondered whether targeted insertion at transcriptional start site would also change lncRNA expression.

CRISPR/SaCas9-mediated stimulation of homologous recombination was utilized to facilitate faithful targeted insertion.

Of the three models of protein localization in bacterial cells cited (Fig. 8), namely targeted insertion, selective degradation, and diffusion/capture31, our results revealed that CadC is localized by a diffusion/capture mechanism dependent on the DNA.

Targeted insertion of an exon 1 13 minigene into CYBB exon 1 resulted in no detectable gp91phox expression or ROS activity in iPSC-derived granulocytes.

This demonstrates the efficacy of two correction strategies: seamless repair of specific CYBB mutations by exon replacement or targeted insertion of an exon 2 13 minigene to CYBB exon 2 while retaining exon/intron 1.

Targeted insertion inside transcriptional termination site is suitable for lncRNA tagging.

Red arrow pair shown here represents primers used for PCR confirmation of targeted insertion.