Sentence examples for targets immobilized from inspiring English sources
With this method, amplification and detection are achieved by covalently coupling photoinitiator molecules to streptavidin proteins followed by binding onto biotinylated oligonucleotide targets immobilized to a biosensor surface.
For peptide/protein targets, immobilized metal affinity chromatography (IMAC) resins are frequently used.
Thanks to the design of the internal microfluidic cartridge of the instrument, the selection was performed during the dissociation phase of the SPR analysis by recovering the aptamer candidates directly from the target immobilized onto the sensor chip surface.
The oligonucleotide library was modified through amplifications of random aliquots of the whole combinatorial library, and their end effect was measured by assessing the net amount of oligonucleotides bound to target immobilized with nitrocellulose.
The performance was strongly dependent on the amount of target immobilized onto the resin and the flow rates for both the loading and washing steps.
In this assay, in vitro translated ligands generate a signal by simultaneously binding to a target immobilized on a magnetic bead and to a sensor surface in a commercial acoustic sensing device.
In practice, such a PMFS and the target immobilized on magnetic particles are mixed to initiate competitive binding; target-ligand complexes in equilibrium are recovered via magnetic force; bound ligands are extracted with suitable solvent(s) and concentrated according to a preset concentration ratio.
When targets in solution pass through the flow cell, the binding between targets and immobilized MREs cause a change in mass on the sensor chip surface and is subsequently translated into a change in the refractive index.
These data suggest the targets were immobilized successfully.
Each of the four protein targets were immobilized onto their respective resins at 0.6 μg/μL.
Targets were immobilized onto Ni-NTA agarose or amine-functionalized polystyrene TentaGel beads.
