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These data and data from transient trasfection experiments, showing that miR-20a over-expression induces a greater percentage of senescent cells than si-LRF (Figure 2e), indicate that although LRF is target of miR-20a, the modulation of other targets contributes to the senescent effect.
Thus, it is critical to further dissect how the derepression of these targets contributes to the observed actin defects.
The ability of the virus to colonize diverse cellular and tissue targets contributes strongly to persistence in the setting of HAART, and is responsible in part for the emergence of antiretroviral drug resistance.
Strohmaier et al. provide strong evidence that abrogation of hCdc4-mediated degradation of cyclin E, and perhaps other targets, contributes to development of some breast cancers, underscoring the important role played by Cdk deregulation in this tumor type.
Again, with in vitro studies, which should not take so long to perform, further dissection could be done of how the derepression of actin targets contributes to the observed actin defects.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com