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miR-210 targets analysis was performed with MiRonTop (available at http://www.microarray.fr:8080/miRonTop/index) as previously described.
CH selected the training group for the CART method, gene ontology analysis, new targets analysis and drafted the manuscript.
We next performed microRNA targets analysis of the differentially expressed genes for further investigating the posttranscriptional control of them.
In an effort to uncover the potential regulatory mechanism underlying the transcription of the cadmium modulated gene sets, we performed transcription factor binding sites analysis within the promoters and microRNA targets analysis of the cadmium modulated genes.
Using the KEA kinase targets analysis, we found that both EGF and IFNγ induce targets of MAPK-1, -3 and −14, as well as of the receptor kinases EGFR, INSR, JAK-1 and −2.
To elucidate the pathogenic role of the significantly differentially expressed miRNAs at diagnosis and relapse (fold change > 2.0 and P< 0.001), a total of 46 miRNAs were selected for targets analysis.
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X.G. assisted with off-target analysis.
Figure 1: Experiment timeline and target analysis.
It is called Operational Target Analysis and dated January 2010.
Off-target analysis of sgRNA2b-induced human ES cells.
Targeted analysis is now possible to characterize gene families of interest (e.g. transcriptional factors).
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