Sentence examples for targeting to delete from inspiring English sources

Moreover, a paired-guided RNA CRISPR/Cas9 library has recently been generated to screen for lincRNAs by performing multiplex targeting to delete the entire lincRNA sequences (Zhu et al., 2016).

We have used gene targeting to specifically delete PKD alleles in DT40 chicken B cells and can thus use PKD-null DT40 cells to assess the relative contribution of individual PKD isoforms in class II HDAC control versus oxidative stress responses and NFκB regulation in lymphocytes.

In order to inactivate the Ctgf gene, we have made a targeting construct to delete exons 3 through 5 of the Ctgf gene in the genome in ES cells as illustrated in Fig. 1A.

A Southern Blot hybridised with a probe upstream of the 5' homology arm, confirmed accurate insertion of the targeting vector to delete the region encompassing exons 7 8 in SD and F344 clones (Figure 1D).

To generate WNK4 −/ − mice, we designed a targeting vector to delete a single exon (exon 2) of the WNK4.

We used conditional targeting methods to delete the Apc and RBP-J genes simultaneously in the intestinal epithelium of adult mice.

We previously used conditional gene targeting to engineer mice with sperm deleted of the Pol B gene, which encodes Pol β.

The targeting plasmid used to delete hprt exon1 was called pΔHprt to indicate this deletion.

To determine whether CIB1 is required for IGC in DT40, serial gene targeting was used to delete exons 5 and 6 of the CIB1 gene (Fig. 3A).

To determine the influence of CD44 deficiency on CIA, Mikecz and colleagues [ 69] used a targeting vector designed to delete, by homologous recombination, most of exons 4 and 5 in the murine CD44 gene, including a substantial part of the HA-binding domain and the coding site of the IM7.8.1 epitope.

In fact, two independent research groups used mouse gene-targeting techniques to delete ADAMTS-4 or ADAMTS-5.