Steps were taken in targeting the viral injection site to the rostral-lateral portion of the SNc to avoid infection of GABAergic neurons in the VTA or SNr.
Another important factor is properly targeting the site for injection [ 46], especially if the target site has limited space.
We describe a technique for intraspinal injection targeting the lumbar ventral horn in rodents.
LRA techniques involve local anaesthetic injection targeting the brachial plexus, at various levels, and therefore, a temporary blockade of the action potentials conducted by sensory and motor fibres, mainly corresponding to the anterior roots between C5 and C7.
The scalp was exposed and a unilateral injection targeting the SN was preformed at coordinates AP -5.2, ML -2 and DV -7.4 with bregma as a point of reference.
The steps required for this technique are the following (Fig. 3): Open image in new window Fig. 3 Glenohumeral joint injection by targeting the rotator interval.
For distal radioulnar joint arthrography [15, 16] (Fig. 8): Open image in new window Fig. 8 Distal radioulnar joint injection by targeting the posterior radioulnar recess.
Arthrography of the radiocarpal joint can be performed as follows [14] (Fig. 7): Open image in new window Fig. 7 Radiocarpal joint injection by targeting the posterior radioscaphoid recess.
Viral vector models were able to overcome this by targeting the basal ganglia directly through injection into the SN and have been shown repeatedly to produce a progressive nigrostriatal degeneration along with motor impairments.
The technical challenge is successfully targeting the perivitelline space, and avoiding injection directly into the circulatory system.
