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In agreement with reported studies [61], these results indicate that AONs targeting longer target sites can induce exon skipping more effectively and efficiently by possibly binding to the pre-mRNA more stably as compared to AONs targeting shorter target sites.
By contrast, RT-PCR, targeting shorter fragments and using a fluorescent probe, greatly improved the speed, sensitivity and specificity of detection, in particular when coupled to the rational selection of PCR targets in genomic sequences according to the experimental objective and the degree of specificity required (genus-, subspecies-,bspecies-, strain- or gene-specific) [ 15- 17].
Although none of the suggested markers were tested in this study, we found the software MitoMapper (Yang et al., 2011) was readily applicable to our xenopus data, yielding either suites of primer pairs to generate overlapping amplicons for complete de novo mtDNA coverage, or primer pairs for targeting shorter gene/genome regions (data not shown).
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Non-targeting or CYCLON targeting short hairpin (sh) RNA sequences were designed using the DSIR algorithm (http://biodev.cea.fr/DSIR/DSIR.html) (Vert et al, 2006).
Twenty of the most important tick hosts were grouped taxonomically and six group-specific primer sets, targeting short mitochondrial DNA regions, were designed de novo.
Introduction: Targeting short peptides are gaining interests in the molecular imaging field.
Though Anchor had been targeting short-form audio, professional podcasters began using its app in greater numbers last year to take advantage of several of its tools.
Targeting short sleep duration may offer a novel and effective method of preventing and treating obesity [ 11].
For example, rational design can consider targeting short helical regions to induce refolding to an inactive conformation.
The presence of EBLV RNA (03011FRA) was determined by reverse transcription PCR (RT-PCR) targeting short viral gene regions (5 ).
A single 60-mer probe was designed to target shorter sequences (60∼150 bps) following the best probe methodology.
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