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Indeed it is tempting to envision that, in a context with soft-targeting rules and pervasive dual targeting of proteins, the retargeting of multiple inter-related enzymes such as those acting on the same pathway may have been more plausible.
One such tactic is the targeting of proteins involved in intracellular signal transduction.
Monomeric fluorescent proteins of different colors are widely used to study behavior and targeting of proteins in living cells.
A number of cellular transport processes support this organization, including active targeting of proteins and passive corralling between compartments.
This compartmental structure generates a new concept in cell biology compared with the simpler prokaryotic cell structure, namely the specific targeting of proteins to intracellular compartments.
In order to study the targeting of proteins to these specialized secretory organelles, we have engineered green fluorescent protein (GFP) fusions to the rhoptry protein ROP1 and the microneme protein MIC3.
Eukaryotic linear motifs (ELMs) are short stretches of amino acids often located within intrinsically disordered regions, which have been shown to help the targeting of proteins to specific subcellular localizations, determining the modification state of proteins, or regulating protein activity in a context-dependent manner18.
This method allowed: (i) subcellular targeting of proteins in differentiating Z. elegans cells; (ii) co-expression of several reporter genes; (iii) promoter activity monitoring; and (iv) gene silencing by the direct introduction of double-stranded RNAs.
The best-known function of ubiquitilation is the targeting of proteins for degradation by the proteasome.
CMA involves the selective targeting of proteins containing a KFERQ peptide motif to lysosomes.
Analysis of L. donovani GLOI sequences with MitoProt II [9] suggested targeting of proteins to the mitochondria.
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